A Secret Weapon For attune flow cytometer
A Secret Weapon For attune flow cytometer
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At that time, the early indications have been dismissed as particles, although the retrospective analysis shown shared properties While using the problematic cells in tradition. Tracing the infection assisted the lab establish supplemental laboratory strategies for screening and defense of assay-critical cell strains.
As samples are acquired about the Attune CytPix Flow Cytometer, the substantial-pace brightfield digicam captures and outlets visuals of detected events in a rate around six,000 pictures for every next, with regards to the flow price and impression measurement. For greater adaptability, Attune Cytometric Software lets you adjust the impression capture frequency as needed and choose a selected gate for impression seize, if demanded.
Mix acoustic concentrating technology with substantial-parameter Evaluation for versatile spectral unmixing and conventional workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating that has a higher-pace brightfield camera for simultaneous higher throughput flow cytometry and substantial resolution brightfield imaging.
"A picture isn't fairly worth 1000 dots, however it seriously really helps to see photographs. It is great to find out pictures that correlate with staining. One example is, AnnexinV-favourable cells which can be morphologically different than healthful cells, or CD14+ monocytes that happen to be larger sized and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Moreover, it's illuminating to discover the number of doublets slip through classic singlet gates, and wonderful to verify the status of sudden double-positive functions in my Evaluation."
Pair your Attune flow cytometer having an autosampler for elevated workflow performance, and insert robotic integration for optimum throughput.
Our experts will design panels for you as often times you need—cost-free, no products buy desired.
The Attune NxT cytometer is the reputable workhorse instrument your lab can count on for regular and trustworthy functionality. The Attune CytPix design contains a substantial-pace brightfield digital camera and AI-pushed graphic Assessment application, with the latest updates including machine Discovering applications and an check here intuitive consumer interface, enabling Increased facts precision, accuracy, and speedier insights into sample biology.
Consumers can perspective occasion photographs in actual-time to confirm sample top quality, optimize protocols, and report the morphology of each mobile population determined for analysis. Imaging seize also Advantages from acoustic concentrating, which helps you to placement the cells to make sure that a pointy, centered graphic is acquired.
AI-driven automatic picture Investigation Together with the Attune Cytometric Software package translates function functions into unique morphology parameters that can be coupled with common fluorescence and scatter parameters to reveal hugely reproducible inhabitants alterations and one of a kind subsets.
We previously shown the strength of imaging Auto-T/Ramos mobile interactions. Allow’s evaluate just the populace of best fascination, the double beneficial occasions, to learn more. We can now use prolonged morphological parameters (here, circularity and skewness of depth) to further analyze the characteristics of these populations and refine gating on these situations, increasing info robustness.
Built-in dimensionality reduction tools improved resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to verify anticipated population resolution in lysed total human blood. User can look at events from Every single cluster for visual affirmation of cell phenotypes.
Flexible payment prepare from Agilent Financial Methods help you have the essential instrumentation you will need for immediate job, or to improve your lab.
At the heart of each Attune Flow Cytometer lies our revolutionary acoustic concentrating technological know-how, which makes use of sound waves to meticulously align cells within the flow cell. This ground breaking tactic surpasses regular hydrodynamic solutions, causing speedier processing speeds, Remarkable information top quality, and amplified sample throughput.
Since the cell line had Earlier been analyzed about the Attune CytPix Flow Cytometer, the scientists went back to the pictures and had been capable to doc the microbial an infection not less than 5 times previously.
The Attune CytPix Flow Cytometer will allow users to visualise samples throughout acquisition, validate the accuracy of gating methods, and aid benefits with reputable documentation for publication reasons.
What seemed to be a homogeneous populace is definitely more varied—an insight that should be regarded when interpreting effects.